{"id":4245,"date":"2021-07-26T12:30:02","date_gmt":"2021-07-26T12:30:02","guid":{"rendered":"https:\/\/healthycyte.com\/?p=4245"},"modified":"2021-07-26T12:30:02","modified_gmt":"2021-07-26T12:30:02","slug":"pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses","status":"publish","type":"post","link":"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/","title":{"rendered":"PCR (Polymerase Chain Reaction) Introduction, History, Steps, Components, types, and Uses"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_69_1 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #1e73be;color:#1e73be\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #1e73be;color:#1e73be\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 eztoc-toggle-hide-by-default' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#Introduction\" title=\"Introduction:\">Introduction:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#History\" title=\"History:\">History:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#Components_of_PCR\" title=\"Components of PCR:\">Components of PCR:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#PCR_Steps\" title=\"PCR Steps:\">PCR Steps:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#Types_of_PCR\" title=\"Types of PCR:\">Types of PCR:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#Uses_of_Polymerase_chain_reaction\" title=\"Uses of Polymerase chain reaction:\">Uses of Polymerase chain reaction:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#FAQs\" title=\"FAQs:\">FAQs:<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#What_does_PCR_stand_for\" title=\"What does PCR stand for?\">What does PCR stand for?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#Is_PCR_test_natural\" title=\"Is PCR test natural?\">Is PCR test natural?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#What_is_rt_PCR\" title=\"What is rt PCR?\">What is rt PCR?<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#Doctors_opinion_about_PCR\" title=\"Doctor\u2019s opinion about PCR\">Doctor\u2019s opinion about PCR<\/a><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"Introduction\"><\/span>Introduction:<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400;\">Nowadays<\/span><b>,\u00a0<\/b><span style=\"font-weight: 400;\">Earth is affected by a new type of virus called Covid 19. This virus has shocked the whole world with its pandemic spread. But why I am talking about this pandemic situation is, whenever someone is facing flu-like symptoms so the doctor advised a Diagnostic test called ad\u00a0<\/span><b>PCR<\/b><span style=\"font-weight: 400;\">. You all are aware of this test or the word \u201cPCR\u201d.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">A lay man just knows the word but he always Questions\u2019s what a PCR is? PCR is an abbreviation of\u00a0<\/span><b>Polymerase chain<\/b><span style=\"font-weight: 400;\">\u00a0<\/span><b>reaction<\/b><span style=\"font-weight: 400;\">\u00a0which is a technique used to amplify DNA into its multiple copies. So we will see the different components and steps involved in this Diagnostic Test.<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"History\"><\/span>History:<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400;\">Back in 1976, the enzyme\u00a0<\/span><b>Taq DNA Polymerase<\/b><span style=\"font-weight: 400;\">\u00a0was discovered with a help of\u00a0<\/span><b>Thermus aquaticus<\/b><span style=\"font-weight: 400;\">. (Bacteria). This means that scientists were very keen that we have now a thermostable enzyme that can give a lot of advancement in the field of Molecular biology. After this\u00a0<\/span><a href=\"https:\/\/en.wikipedia.org\/wiki\/Kary_Mullis\"><span style=\"font-weight: 400;\">Kary mulis<\/span><\/a><span style=\"font-weight: 400;\">\u00a0who was a biochemist in California discovered the new technique with the help of Taq DNA polymerase.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">This technique was named\u00a0<\/span><b>POLYMERASE CHAIN REACTION\u00a0<\/b><span style=\"font-weight: 400;\">(PCR) as the name indicates the use of polymerase that is used in the repeating process. This technique was later modified by other scientists and developed new types. In modern diagnosis, PCR is considered one of the most important techniques in the laboratory<\/span><span style=\"font-weight: 400;\">.\u00a0<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Components_of_PCR\"><\/span>Components of PCR:<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400;\">Components of PCR reaction are referred to as material that is used to do a simple PCR reaction. PCR is a chain reaction means once the material is used at the start and the whole process is done automatically. Let\u2019s now overview the basic components of PCR reaction.<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Its starts with the double-stranded template DNA that includes the target Sequence.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Primers (short single-stranded DNA sequences that are complementary to DNA sequences).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">dNTPs.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">DNA polymerase.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Buffer solution.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Incubators (today automatic thermocyclers are used).<\/span><\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"PCR_Steps\"><\/span>PCR Steps:<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><img decoding=\"async\" loading=\"lazy\" src=\"https:\/\/healthycyte.com\/wp-content\/uploads\/2021\/07\/Double-stranded-DNA-1.png\" alt=\"Double stranded DNA (1)\" width=\"1400\" height=\"540\" class=\"alignnone size-full wp-image-4257\" srcset=\"https:\/\/healthycyte.com\/wp-content\/uploads\/2021\/07\/Double-stranded-DNA-1.png 1400w, https:\/\/healthycyte.com\/wp-content\/uploads\/2021\/07\/Double-stranded-DNA-1-300x116.png 300w, https:\/\/healthycyte.com\/wp-content\/uploads\/2021\/07\/Double-stranded-DNA-1-1024x395.png 1024w, https:\/\/healthycyte.com\/wp-content\/uploads\/2021\/07\/Double-stranded-DNA-1-768x296.png 768w\" sizes=\"(max-width: 1400px) 100vw, 1400px\" \/><\/p>\n<blockquote align=\"center\";><p>Main steps in the polymerase chain reaction (PCR)<\/p><\/blockquote>\n<p><span style=\"font-weight: 400;\">PCR is a technique used to produce a million copies of a specific sequence of DNA that is required. There are three steps with sometime 4 to start a PCR reaction. These steps are important so all of the steps will be discussed separately.<\/span><\/p>\n<ol>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Denaturation<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Annealing<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Extension<\/span><\/li>\n<\/ol>\n<p>\n<b><\/b><\/p>\n<ul>\n<li aria-level=\"1\"><b>Denaturation:<\/b><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">In this first step of PCR, the temperature is increased above 94 to 95 degrees Celsius. This temperature increase causes the breakage of the Hydrogen bond between DNA base pairs. When the complementary base pairs are separated, this causes the Separations of DNA to double helix structure (denature).<\/span><\/p>\n<p>\n<b><\/b><\/p>\n<ul>\n<li aria-level=\"1\"><b>Annealing:<\/b><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">In the second step, Primer is added into already denatured DNA. The temperature is lowered up to 50-65 degree Celsius. This lower temperature causes the primer to bind or anneal on the specific site of DNA. Primer binds to the bases that are present in DNA. This process is known as annealing.<\/span><\/p>\n<p>\n<b><\/b><\/p>\n<ul>\n<li aria-level=\"1\"><b>Extension:<\/b><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">This is the most step as it makes new complementary strands of DNA. Temperature increases to 70 to 75 degrees Celsius. The extension of the complementary strand needs a DNA polymerase enzyme. This enzyme needs a specific temperature that uses dNTPs to form a new strand. This process is called Extension.<\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" src=\"https:\/\/healthycyte.com\/wp-content\/uploads\/2021\/07\/Cycle-1.png\" alt=\"copies of DNA\" width=\"1000\" height=\"900\" class=\"alignnone size-full wp-image-4259\" srcset=\"https:\/\/healthycyte.com\/wp-content\/uploads\/2021\/07\/Cycle-1.png 1000w, https:\/\/healthycyte.com\/wp-content\/uploads\/2021\/07\/Cycle-1-300x270.png 300w, https:\/\/healthycyte.com\/wp-content\/uploads\/2021\/07\/Cycle-1-768x691.png 768w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/><\/p>\n<blockquote align=\"center\";><p>Polymerase chain reaction (PCR) produces lots of copies of DNA<\/p><\/blockquote>\n<p><b>This cycle is repeated many times and forms millions of copies of DNA.\u00a0<\/b><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Types_of_PCR\"><\/span>Types of PCR:<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400;\">PCR has a lot of new types that are making molecular biology easier to understand. Following are some types that are discovered.<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Real-time PCR (qPCR or Quantitative PCR):<\/b><span style=\"font-weight: 400;\">\u00a0<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">Most used type of PCR, even its available in the small diagnostic laboratory. This is the type in which amplification of DNA and also numbering of specific DNA is done. This is done using fluorescent dyes which are available commercially<\/span><span style=\"font-weight: 400;\">.<\/span><span style=\"font-weight: 400;\"> For example cloning of virus such as Dengue virus and Hepatitis C virus. Cloning of Bacteria such as Salmonella Typhi Etc.<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Reverse transcriptase PCR (RT PCR):<\/b><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">As the name indicates, this type of PCR involves Reverse Transcriptase (RT). That why this PCR is used for the amplification of RNA. Reverse transcriptase helps to form cDNA from RNA, which is then used for PCR amplification.\u00a0<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Multiplex PCR:<\/b><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">The modified type of PCR in which multiple specific target sequences are amplified using multiple primers in a single PCR mixture. It means that in a single time we can amplify multiple needed sites, instead of running them in separate amplification. This is cost-effective PCR.<\/span><\/p>\n<p>\n<b><\/b><\/p>\n<ul>\n<li aria-level=\"1\"><b>Nested PCR<\/b><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">\u00a0Used where you need two sets of new primers used against the same targeted DNA. This type of PCR is specific for the target DNA and has low sensitivity. These are also cost-effective assets of primer that are used at a single time.<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Uses_of_Polymerase_chain_reaction\"><\/span>Uses of Polymerase chain reaction:<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ol>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">One of the most important applications of Using PCR is\u00a0<\/span><b>GENE EXPRESSION<\/b><span style=\"font-weight: 400;\">. Real-time PCR is used for gene expression. We can also determine the change of expression in any cell, tissue and, molecule morphology.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">PCR is also used in cloning. PCR is generally utilized in cloning DNA (Deoxyribo Nucleic Acid) parts of interest, in a method known as\u00a0<\/span><b>PCR cloning<\/b><span style=\"font-weight: 400;\">.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">It is also used to detect simple sequencing of DNA to read.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">We can also do\u00a0<\/span><b>gene isolation<\/b><span style=\"font-weight: 400;\">\u00a0through PCR. It is done by selective DNA amplification on a specific site.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Polymerase chain reaction is also used to\u00a0<\/span><b>detect genetic changes or variations<\/b><span style=\"font-weight: 400;\">\u00a0in different types of allele.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">We can also add plasmids or add a special type of mutation in a specific cell with the help of using PCR.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">PCR is one of the most important techniques nowadays used in the Field of Forensic, clinical bacteriology, virology and, clinical diagnostic.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">It is also used in agriculture biotechnology for detecting Plants genotyping for breedings. Etc.<\/span><\/li>\n<\/ol>\n<h2><span class=\"ez-toc-section\" id=\"FAQs\"><\/span>FAQs:<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"What_does_PCR_stand_for\"><\/span>What does PCR stand for?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>PCR stands for polymerase chain reaction. It is the diagnostic test used to amplify DNA into its multiple copies. Today it is used in both research and diagnoses.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Is_PCR_test_natural\"><\/span>Is PCR test natural?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400;\">Yes PCR test has all techniques following the natural process such as transcription and translation. Only uses the commercially available products.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"What_is_rt_PCR\"><\/span>What is rt PCR?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400;\">Reverse transcriptase PCR (RT PCR) is a type of PCR that uses reverse transcriptase enzyme for the amplification of RNA. A cDNA is produced with the help of RT from RNA to form multiple copies of RNA.<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Doctors_opinion_about_PCR\"><\/span>Doctor\u2019s opinion about PCR<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The importance of PCR recommendation by the doctors over\u00a0<b>the ELISA<\/b><span style=\"font-weight: 400;\">\u00a0test is due to the sensitivity and specificity of this test. According to\u00a0<\/span><a href=\"https:\/\/www.tdlpathology.com\/covid-19\/covid-19-pcr-assay\/\"><span style=\"font-weight: 400;\">the Doctors Laboratory<\/span><\/a><span style=\"font-weight: 400;\">\u00a0UK, Covid PCR gives 98 and 100% of PCR sensitivity and specificity. This shows the actual numbering as\u00a0<\/span><a href=\"https:\/\/www.who.int\/news\/item\/20-01-2021-who-information-notice-for-ivd-users-2020-05\"><span style=\"font-weight: 400;\">WHO also recommended nucleic acid testing<\/span><\/a><span style=\"font-weight: 400;\">\u00a0of PCR for detecting Covid Variant. They also recommend to Healthcare workers to give PCR results with consideration of time, used sample, clinical observation and, epidemiological information. Today PCR is used in every diagnostic laboratory all over the world.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Introduction: Nowadays,\u00a0Earth is affected by a new type of virus called Covid 19. This virus has shocked the whole world with its pandemic spread. But why I am talking about this pandemic situation is, whenever someone is facing flu-like symptoms &#8230; <\/p>\n<p class=\"read-more-container\"><a title=\"PCR (Polymerase Chain Reaction) Introduction, History, Steps, Components, types, and Uses\" class=\"read-more button\" href=\"https:\/\/healthycyte.com\/pcr-polymerase-chain-reaction-introduction-history-steps-components-types-and-uses\/#more-4245\">Continue Reading&#8230;<span class=\"screen-reader-text\">PCR (Polymerase Chain Reaction) Introduction, History, Steps, Components, types, and Uses<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":4248,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_newsletter_tier_id":0,"two_page_speed":[]},"categories":[1],"tags":[681,682,683],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/healthycyte.com\/wp-content\/uploads\/2021\/07\/PCR-Polymerase-Chain-Reaction.jpg","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/healthycyte.com\/wp-json\/wp\/v2\/posts\/4245"}],"collection":[{"href":"https:\/\/healthycyte.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/healthycyte.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/healthycyte.com\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/healthycyte.com\/wp-json\/wp\/v2\/comments?post=4245"}],"version-history":[{"count":0,"href":"https:\/\/healthycyte.com\/wp-json\/wp\/v2\/posts\/4245\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/healthycyte.com\/wp-json\/wp\/v2\/media\/4248"}],"wp:attachment":[{"href":"https:\/\/healthycyte.com\/wp-json\/wp\/v2\/media?parent=4245"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/healthycyte.com\/wp-json\/wp\/v2\/categories?post=4245"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/healthycyte.com\/wp-json\/wp\/v2\/tags?post=4245"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}